Apheresis in Korean Red Cross Nambu Blood Center / 대한수혈학회지

BACKGROUND: The yields of plateletpheresis products are important to meet standard platelet transfusion doses. The use of single donor platelets (SDPs) has been increased substantially worldwide. Korean Red Cross decided to supply and started collection of SDPs since January, 2000. This study evaluates platelet yields of plateletpheresis and plasmapheresis of Korean Red Cross Nambu blood center. METHODS: The records for SDPs collected by Amicus(TM) (Baxter, Deerfield, IL, USA), MCS+ (Haemonetics, Braintree, MA, USA) and Trima (Gambro BCT, Lakewood, USA) between Jan. 2005 and Nov. 2005 were evaluated. The records for plasma collected by Autopheresis-C(TM) (Baxter,Deerfield, IL, USA), MCS(TM), PCS 2 (Haemonetics, Braintree, MA, USA) between Jan. 2005 and Nov. 2005 were also evaluated. RESULTS: Platelet yields of SDP less than 3 x 10(11) accounted for 6.45% of all collections. The rate of SDPs which platelet yields less than 3 x 10(11) collected by AMICUS is 4.84%, MCS+ is 7.72%, TRIMA is 7.01%. The rate of plasmapheresis products under the criteria is 0.7%. Plasma collected with Autopheresis-C(TM) made a concern about break down of filtration pore and occuring of hemolysis during apheresis procedures. CONCLUSION: Platelet yields less than 3 x 10(11) accounted for 6.45% of all collections. The rate of plasmapheresis products under the criteria is 0.7%. This study demonstrated that qualfied management and thorough understanding of the plataletpheresis technology are necessary to increase productivity of SDPs with platelet yields 3x1011 or over.

Cryopreservation and Thawing of Red Blood Cells Using Haemonetics ACP 215 / 대한진단검사의학회지

BACKGROUND: The FDA has approved the storage of frozen red blood cells (RBCs) at -80degrees C for 10 years. After deglycerolization, the RBCs can be stored at 4degrees C for no more than 24 hours, because open systems are currently being used. We evaluated Haemonetics ACP 215, an automated, functionally closed system, for both the glycerolization and deglycerolization processes. METHODS: Thirty packed RBCs that had been glycerolized and stored at -80degrees C for 2 weeks were thawed, deglycerolized and resuspended in AS-3. The RBCs were then stored at 4degrees C for 2 weeks. For the evaluation of the procedure, RBC recovery rate, osmolarity, specific gravity, LDH, K+, Hb-2, 3 DPG, Hb-ATP, and plasma hemoglobin were tested at day 0 and day 14. RESULTS: The recovery rate of RBCs was 83.7+/-2.6% (78.9-88.8%). The Hb ATP and 2, 3-DPG of RBCs were 5.16+/-1.0 mol/g Hb and 10.4+/-2.4 mol/g Hb, respectively, at day 0. The supernatant K+, specific gravity, osmolarity, LDH were 1.3+/-0.6 mmol/L, 1.008+/-0.001, 295.0+/-3.1 mOsm/kgH2O, 175.0+/-39.0 unit/L, respectively. All measurements were acceptable to allow the RBCs deglycerolized on ACP 215 to be stored at 4degrees C for 14 days. The blood cultures were negative at day 0 and day 14. CONCLUSIONS: Haemonetics ACP 215 provides a closed, automated system for RBC glycerolization and deglycerolization. This study showed that the RBCs that were glycerolized and deglycerolized in the automated instrument and stored in AS-3 at 4degrees C for 14 days are of an acceptable quality.

ABO Gene Analysis of Discrepant ABO Blood Group in Blood Donors / 대한수혈학회지

BACKGROUND: An exact ABO blood group is essential for prevention of transfusion accident and safe transfusion therapy. It is known that one of causes of ABO discrepancies is ABO subgroup caused by genetic polymorphism. Therefore, we analyzed ABO genotype of ABO discrepancies in blood donors and studied the distribution and cause of ABO discrepancies. METHODS: This study examined 118 samples showing ABO discrepancies of ABO blood typing between May 2003 and Dec 2003. ABO genotyping using the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method was performed on 118 samples. Restriction enzymes including BssH II, Kpn I and Alu I were used for PCR-RFLP. RESULTS: The genotypes of 118 cases were composed of 43 cases of A/B, 12 cases of A/O, 10 cases of B/O, 1 case of B/B, 37 cases of cis-AB/O, 4 cases of cis-AB/A, 11 cases of cis-AB/B. The genotype of cis-AB/O showed 32 cases with phenotype A2 B3 , 2 cases with phenotype A2 B, 2 cases with phenotype A1 B3 , 1 case with phenotype Ael B. The genotype of cis-AB/B showed 11 cases with phenotype A2 B, and cis-AB/A showed 2 cases with phenotype A2 B3 , 1 case with phenotype A1 Bx and 1 case with phenotype A1 Bel. CONCLUSION: These data demonstrated that the most frequent genotype of ABO discrepancies in our study is cis-AB. The most predominent phenotype of cis-AB/O is A2 B3 . ABO genotyping is useful in resolving ABO discrepancies, and determination of ABO subgroups.